Screening of marine sponge-associated bacteria from Echinodictyum gorgonoides and its bioactivity

Abstract

This paper describes the evaluation of bacterial strain (MB2) bioactivity isolated from marine sponge. The sponge Echinodictyum gorgonoides associated bacterial strain MB2 was tested for its action against various human pathogenic bacterial isolates. The biochemical tests were done to determine the characterization of the bacterial strain and to identify the specific MB2 strain. The cytotoxicity studies of the bacterial strain (MB2) isolated from the sponge was analysed using the Brine shrimp cytotoxicity assay and confirmed against the glioma C6 cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The availability of proteins were analysed by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Finally, the isolated bacterial MB2 strain from the marine sponge E. gorgonoides was identified through means of 16sRNA sequence analysis. It was then confirmed by means of basic local alignment search tool (BLAST). From these results, it is confirmed that the strain MB2 is Staphyloccous sp. Hence it is assumed that the sponge associated proteins and the presence of secondary metabolites could be the source for determining various biological activities.

Key words: Echinodictyum gorgonoides, glioma C6 cell lines, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 16s rRNA.