Genetic diversity analysis of stress tolerant rice (Oryza sativa L.)

Abstract

Fourteen rice genotypes, composed of six salt tolerant, three submergence tolerant, two drought tolerant genotypes along with three high yielding genotypes, released from Bangladesh Rice Research Institute (BRRI) were used for genetic diversity analysis using 40 simple sequence repeats (SSR) markers. All of the used SSR markers were found polymorphic among the 14 rice genotypes. The amplicon size ranged from 75 bp (RM436) to 330 bp (RM26360). A total of 168 alleles were detected, the number of alleles per locus ranged from 2 (RM252, S03120) to 6 (RM570, S12055, S11033) with an average of 4.2 alleles per locus. Polymorphic information content (PIC) value varied from 0.21 (RM252) to 0.76 (S07024) with an average of 0.57. From genetic distance co-efficient, the highest and lowest genetic distant varieties were found for BRRI dhan28 vs. BRRI dhan43 (0.82%) and BRRI dhan40 vs. BRRI dhan44 (0.37%) respectively. Unweighted pair group method with arithmetic mean (UPGMA)-cluster analysis divided the rice genotypes into four distinct clusters. The information obtained from this study would be useful for planning the breeding program to develop stress tolerant rice variety with high yielding ability and fine grain quality.

Key words: Genetic diversity, simple sequence repeats (SSR) markers, stress tolerant, rice.