Effectiveness of tissue culture media components on the growth and development of cauliflower (Brassica oleracea var. Botrytis) seedling explants in vitro

Abstract

A key factor in the application of in vitro techniques to cauliflower improvement is the development of efficient protocols for regeneration of plants from tissue for use in breeding programs for the selection of the desirable genotypes under biotic and abiotic stress. Experiments were conducted to study the effect of different media components (agar or agar + sucrose or agar + Murashige and Skoog (MS) salts or agar + sucrose + MS) on callus induction and regeneration from five explants types (cotyledon, hypocotyls, shoot apex, primary root and root tip) and also the effects of auxin and cytokinin were carried out using one F₁ hybrid cauliflower cv. Medallion. The results show that cotyledons, mid roots and root apices grown on agar + MS + sugar were the most developed compared with explants on the other media. This medium was the most productive in terms of lateral root number and root length. The presence of 2,4-dichlorophenoxyacetic acid increased callus production compared to 6-benzylamino purine. Within the selected explants, a significant difference was indicated between different explants under different treatments. Liquid culture was more successful at producing viable plantlets than solid culture.

Key words: Cauliflower, explants, in vitro, growth regulator, growth characters, solid and liquid culture.