Effect of AgNO3 on androgenesis of Brassica oleracea L. anthers cultivated in vitro

Abstract

The present article is a synthesis study accomplished at Vegetable Research and Development Station Bacau regarding the implication of silver nitrate (AgNO₃) in modulating the morphogenetic reaction of white cabbage anthers cultivated in vitro. According to literature, silver nitrate is a strong inhibitor of ethylene action. Embryo formation, shoot generation and rooting of plantlets are key points on which relies the entire concept of in vitro tissue culture. Silver ions, in the form of nitrate play an important role in promoting the somatic embryogenesis and organogenesis, which led its wide spread use in different plant tissue culture in vitro. Therefore, our researches focused on testing different concentrations of AgNO₃ (5, 10, 15, 25, 30, 50 and 60 μM) added to a pre-tested medium formula– Murashige and Skoog (MS), 1962 basal medium supplemented with benzylaminopurine (BAP)- 8.9 μM and naphthylacetic acid (NAA) 2.7 μM. The biological material is represented by unopened flower buds containing anthers with microspores at late uninucleate to binucleate stage. In the culture condition investigated in this study, the data obtained promotes the utilization of AgNO₃ in a concentration of 50 μM for the successfully induction and sustaining of regenerative processes of white cabbage anthers cultivated in vitro.

Keywords: Buds, embryogenesis, ethylene, organogenesis, regeneration