Cloning and analysis of plant fatty acid desaturase 7 gene promoter from Brassica napus

Abstract

In order to investigate the regulation mode of Brassica napus FAD7 gene in response of thermal stress, we measured the protein levels of BnFAD7 in plant at low and high temperature, and then analyzed promoter activity of 5’-flanking regions of BnFAD7 by transient gene expression in B. napus protoplasts at different temperatures. Our studies indicated that no significant change occurred in the expression level of BnFAD7 both at high and low temperature, while BnFAD7 promoter showed a heat-induced regulation mode and slowly increased activity at the chilling conditions, which suggested there are heat-induced cis-action element lies in BnFAD7 promoter sequence. Our data also suggested that a post-transcription regulation pattern existed to ensure BnFAD7 function in the acclimation to temperature stress. Furthermore, our studies give new evidence for the hypothesis that BnFAD7 and BnFAD8 gene may come from the same ancestor gene.

Keywords: Brassica napus, fatty acids desaturase, promoter analysis, transient expression, BnFAD7