A simple and rapid lysis method for preparation of genomic DNA from Gram-negative bacteria

Abstract

A very efficient, simple and economic lysis method for rapid preparation of genomic DNA from Gramnegative bacteria was developed. This method includes a novel step lysis by treatment with carvacrol followed by simple ethanol precipitation. The procedure was realised without using of detergents or enzymes. Moreover, the resultant genomic DNA was in good quantity and quality and can be used successfully for restriction endonucleases digestion, PCR amplification and others types of molecular biology manipulations.

Keywords: Genomic DNA, lysis, carvacrol, Gram-negative bacteria, Escherichia coli, Erwinia chrysanthemi