Mapping of randomly amplified polymorphic DNA primer (RAPD) on chromosome 2A of common wheat

Abstract

Randomly amplified polymorphic DNA primers (RAPDs) were mapped on chromosomes 2A of wheat genotypes using “Chinese Spring” nullisomic-tetrasomic lines. One particular pair of chromosome was absent and another homologous pair was present in the extra dose. Genomic DNA was isolated from two genetic stocks viz; NT1D1B and NT2A2B. Polymerase chain reaction (PCR) were conducted using RAPD primers GLC-07 and GLB-11. PCR amplification using primer GLC-07 produced single band of approximately 1100 bp in both the genetic stocks, indicating that the primer was annealed to a loci other than chromosome 2A. RAPD primer GLB-11 amplified a polymorphic allele of approximately 500 bp which was present in NT1D1B but was absent in NT2A2B, indicating that the locus was present on chromosome 2A of common wheat. Hence, this marker (GLB-11) can reliably be used to keep track of chromosome 2A of wheat.

Key words: Wheat, randomly amplified polymorphic DNA primer (RAPD), mapping, chromosomes, homologous, genomes.