Influence of kinetin on in vitro rooting and survival of banj oak (Quercus leucotrichophora L.)

Abstract

A study concerning the influence of cytokinins on shoot regeneration by using different stem segments derived from in vitro raised seedlings and their subsequent rooting was conducted in banj oak (Quercus leucotrichophora L.). Cytokinins play an important role in shoot regeneration and their multiplication. In the present study, cytokinins particularly kinetin (Kn) influenced in vitro rooting and subsequent survival of these in vitro raised plants in addition to shoot multiplication. It was found that the microshoots raised via kinetin treatment rooted very well (94.44%) without any basal callus formation in comparison to microshoots raised via other cytokinin treatments (that is, BA and 2-iP). In addition to this, these in vitro raised plants showed maximum survival rate (90%) during hardening process. On the basis of available literature this is a unique and significant study regarding the comparative effect of different cytokinins on in vitro propagation study of Q. leucotrichophora by using different stem segments, particularly the influence of kinetin in vitro rooting and survival of in vitro raised plants in addition to shoot multiplication. This significant study could be useful for large scale production of successfully hardened plants so that it would be helpful in conservation of this important Himalayan forest tree species.

Keywords: Quercus leucotrichophora, acclimatization, cytokinins, in vitro rooting