Adhesive ability and biofilm metabolic activity of Listeria monocytogenes strains before and after cold stress

Abstract

Listeria monocytogenes is an important pathogen responsible for major outbreaks associated with food products. Adhesion to surfaces leads to significant modifications in cell physiology. In this work, the ability of L. monocytogenes to produce biofilm and its ability to adhere to abiotic surfaces under cold stress were evaluated. Metabolic activity of biofilm formed by L. monocytogenes before and after cold stress was measured in vitro using the colorimetric method based on the reduction of the tetrazolium salt 2,3-bis(2methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT). The ability to adhere to abiotic surfaces was determined by the ability of the cells to metabolically reduce bromure de 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) to a formazan dye. Our results show that L. monocytogenes strains were able to adhere to abiotic materials with different degrees. In fact, cold stressed strains (-20°C) were more adhesive to polyethylene, glass, polyvinyl chloride and stainless style surfaces than non-stressed cells. Our observations show that the hydrophily varied with cold stress period. At freezing temperature, L. monocytogenes was strongly hydrophobic. Genetic studies of adhesive genes of L. monocytogenes will be required to fully understand the importance of this observation.

Keywords: Listeria monocytogenes, slime production, cold stress, abiotic-surfaces, biofilm formation