Effects of plant growth regulators and photoperiod on in vitro microtuberization of potato (Solanum tuberosum L.)

Abstract

In vitro microtuber production of potato (Solanum tuberosum L.) cvs. Sante and Savalan were studied on solid Murashige and Skoog (MS) basal medium applying different plant growth regulators 2,4-dichlorophenoxyacetic acid and benzylamino purine (2,4-D and BAP) and photoperiods. Cultures were exposed to 16, 8 and 16 h+utter darkness photoperiodic regimes. The experimental design, complete randomized with three replications was applied. The results indicate that the effect of cultivar, hormone and photoperiod significantly had influence on whole traits. Sante cultivar had higher productivity than Savalan for whole measured traits. The results of mean comparison for photoperiod show that highest productivity for whole traits is gained by this treatment 16 h photoperiod+ utter darkness (P₃). In this experiment by using the combination of 2,4-D (2.26 μM) and BAP (22.19 μM), microtubers number, diameter and weight was increased. In this study, the highest number for microtuber in Sante cultivar with hormone 2,4-D and photoperiod P₃ is 9.47 which this high number for Savalan cultivar is gained by using the combination of two plant growth regulators and same photoperiod.

Keywords: Tissue culture, potato, microtuber, photoperiod, hormone