Improvement of electrophoresis performance by spectral analysis

Abstract

This paper describes a new design of standard agarose gel electrophoresis procedure for nucleic acids analysis. The electrophoresis was improved by using the real-time spectral analysis of the samples to increase its performance. A laser beam illuminated the analysed sample at wavelength with the highest absorption of gel components. Thus, DNA band is detected well before it may exits a gel plate. A modified horizontal electrophoresis device was designed, developed and tested.

Keywords: Horizontal electrophoresis, laser, spectral analysis, agarose gel