Halotolerant streptomycetes isolated from soil at Taif region, Kingdom of Saudi Arabia II: RAPD-PCR analysis and salt tolerance-gene isolation

Abstract

The genus Streptomyces is represented in nature by the largest number of species and varieties among the family Streptomycetaceae. This study aimed at extracting the DNA of four halotolerant Streptomyces strains followed by determination of DNA fingerprinting of them using a molecular tool. A trail to isolate salt tolerance gene(s) from their DNA was also aimed. RAPD-PCR technique was applied using seven RAPD-PCR primers. Results show that a total 71 fragments (65 polymorphic and six monomorphic) were amplified from the DNA of the four identified Streptomyces strains. The fragments were divided into polymorphic and monomorphic fragments. Three primers named OPA11, OPB15 and OPC07 did not show any monomorphic fragments. A number of 43 (7, 7, 8, 2, 9, 6 and 4) representing 60.56% were considered as unique DNA markers, and were amplified using OPA11, OPB15, OPC07, OPC18, OPD04, OPE05 and OPO14, respectively. Data shows similarity matrix between the four identified Streptomycs strains based on RAPD-PCR analysis ranged from 21.8 to 40.0. Results show that the P5CR gene was detected in the DNA extracts of three species, namely, Streptomyces cirratus-02; Streptomyces rishiriensis-04; Streptomyces luteogriseus-08, while, mtlD gene was only found in the DNA extracts of S. cirratus-02.

Keywords: Streptomyces, halotolerant, salt tolerance genes, RAPD-PCR, Taif, KSA

African Journal of Biotechnology Vol. 12(13), pp. 1452-1458