Development and characterization of microsatellite loci for Fenneropenaeus penicillatus Alcock

Abstract

Eight novel microsatellite loci from the genome of Fenneropenaeus penicillatus Alcock were developed using the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Thirty (30) wild individuals were used to analyze the polymorphism of these eight microsatellite markers. The results show that the number of alleles per locus and the polymorphism information content ranged from 2 to 7 and from 0.2076 to 0.7484, respectively. The observed and expected heterozygosity were 0.1724 to 0.9130 and 0.1639 to 0.7314, respectively. These microsatellite primers will be used for further population genetic studies, constructing genetic linkage maps or locating quantitative trait locus (QTL) of F. penicillatus Alcock.

Keywords: Genetic markers, Fenneropenaeus penicillatus Alcock, microsatellites