Partial immunity in murine by immunization with a toxoplasmic DNA vaccine

Abstract

Toxoplasma gondii is an obligate intracellular protozoan that is causative agent of atoxoplasmosis, a disease which may result in a spectrum of consequences. Previous studies have reported that DNA vaccine can be effective in partial protection against this parasite. In this study, we constructed a single DNA vaccine containing rhoptry protein 1 (ROP1) and evaluated its immune response in Balb/c mice. We used alum as an adjuvant to enhance the immune response. After intramuscular injection, we evaluated the immune response using cytokine and antibody assay and mortality rate. The results show that mice immunized by pcROP1 with or without alum produced high Th1 immune response compared with the control groups. This kind of DNA vaccine prolonged survival time. The current study showed that ROP1 DNA vaccine could induce partial protective response against toxoplasmosis.

Key words: Toxoplasma gondii, DNA, vaccine, Rhoptry protein 1 (ROP1).