A genetic linkage map of Japanese scallop Mizuhopecten yessoensis based on amplified fragment length polymorphism (AFLP) and microsatellite (SSR) markers

Abstract

A genetic linkage map of the Japanese scallop Mizuhopecten yessoensis was constructed based on 302 markers, including 263 amplified fragment length polymorphism (AFLP) markers and 39 microsatellite (SSR) markers. The two parental maps were constructed according to the double pseudo-test cross strategy with an F₁ progeny of 115 individuals. In the maternal parent, 163 markers were assigned in 20 linkage groups, spanning a total coverage of 2184.9 cM with the average spacing between two adjacent markers was 15.3 cM. In the paternal parent, 155 markers were also mapped into 20 linkage groups, spanning a genetic length of 1882.4 cM with the average marker density of 13.9 cM, respectively. The coverage estimated for the framework maps were 78.3% for the female and 77% for the male without minor linkage groups. Five full alignment linkage groups and four homologous linkage groups could be identified based on the position of 16 high information content SSRs which segregated in the parents. The construction of the M. yessoensis genetic linkage maps here was a part of a genetic breeding program. This linkage map will contribute to the discovery of genes, comparative genomics and quantitative trait loci in Japanese scallop.

Keywords: SSR, AFLP, genetic linkage map, Mizuhopecten yessoensis