Plant regeneration and stimulation of in vitro flowering in Eruca sativa Mill.

Abstract

Explants such as apical buds, axillary buds, cotyledons, cotyledonary nodes, leaves, hypocotyls and immature embryonal axes from in vitro-grown plantlets were inoculated on the Murashige and Skoog (MS) medium supplemented with 4.44 μM 6-benzylaminopurine in combination with 2.85 μM indole-3-acetic acid. Best multiple shoots formation was obtained with cotyledonary nodes. Each inoculated explant produced 18.10 ± 0.66 shoots within 2 to 3 weeks. These shoots were separated carefully and were transferred to the fresh half strength MS solid medium with indole-3-butyric acid (4.90 μM) for the development of the roots. These in vitro-developed plantlets produced flowers on the same medium with supplementation of 6-furfuril kinetin (0.23 μM). These plantlets were successfully transferred to the soil where they grew well for 8 to 10 weeks with 80% survivability.

Keywords: Eruca sativa, cotyledonary nodes, in vitro regeneration, in vitro flowering, shoot multiplication