Efficient callus induction and indirect plant regeneration from various tissues of Jatropha curcas

Abstract

The Jatropha curcas is considered as an important energy plant due to the fact that its seed contains high oil content. Nowadays focus is being placed on J. curcas callus induction and plant regeneration. In this study, explants epicotyl, hypocotyl, petiole and cotyledon of 8-day-old seedlings of J. curcas were utilized for callus induction on media supplied with 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), naphthyl acetic acid (NAA) or indolebutyric acid (IBA) and 0.1 mg/L kinetin (Kin), and the results demonstrated that the combination of 1 mg/L NAA and 0.1 mg/L Kin was the best medium for callus induction and growth. In addition, induced calli were transferred to regeneration medium containing different combination of auxins and cytokinins, and the data showed that the medium containing 1 mg/L thidiazuron (TDZ) and 1 mg/L Kin combined with 0.1 mg/L IBA was propitious to plant regeneration compared with other combinations.

Keywords: Callus induction, indolebutyric acid, Jatropha curcas, kinetin, naphthyl acetic acid, plant regeneration, thidiazuron