Simple sequence repeat (SSR) markers analysis of genetic diversity among Brassica napus inbred lines based on correlation between seed quality traits and seed pigments content

Abstract

Information regarding diversity and relationships among different quality traits and seed coat characterization of breeding material is necessary for hybrid Brassica napus breeding and seed coat color selecting. Simple-sequence repeat (SSR) analysis of the 62 loci distributed uniformly throughout the Brassica napus genome was carried out for 90 inbred lines which were derived from the black-seeded coat and the yellow-seeded coat parents in order to assess genetic diversity among the inbred lines and correlation to different traits with seed coat and seed pigment. The average-linkage (UPGMA) cluster analysis yellow-seeded coat lines and black-seeded coat lines were divided in two major groups; SSR clustering analysis results is linkage with the traits clustering analysis results. The correlation studies showed that seed coat color, anthocyanidin content, total phenol content, melanin content and flavonoid content in seed coat had significant negative correlation with oil content in different environments. The anthocyanidin content, flavonoid content, total phenol content and melanin content had significant positive correlation in seed coat. The results indicate that SSR analysis is effective for the assessment of genetic diversity among Brassica napus inbred lines and seed coat color is one of the most important traits to breeding of B. napus quality improvement.

Keywords: Brassica napus L, quality traits, agronomic traits, correlation analysis