Effectiveness of native Rhizobium on nodulation and growth properties of dry bean (Phaseolus vulgaris L.)

Abstract

This study was conducted in order to evaluate the symbiotic effectiveness of native Rhizobium isolated strains on number of nodule, weight, and morphological properties of dry bean. To realize this purpose, fresh and healthy root nodules were collected from major dry bean-growing districts of Konya, Turkey. These nodules were used to determine the effects of native Rhizobium isolated strains in the experiments. The first experiment was conducted to the determination of the most effective native isolated strains, by using Leonard bottles. Six (1, 3, 5, 23, 69 and 85) isolated strains of 94 native Rhizobium bacteria were found the most effective as compared with reference strain Rhizobium tropici CIAT899. The second experiment was conducted as pot experiment and arranged in a complete randomized block design with three replicates per bacteria. The subjects of the treatment subjects are control, nitrogen control, native isolated strain 1, native isolated strain 3, native isolated strain 5, native isolated strain 23, native isolated strain 69, native isolated strain 85, reference strain (R. tropici CIAT899) and mix strain. It has been shown that the used strains were significantly (P<0.05) increased nodulation and other morphological parameters. Significant differences existed in the symbiotic potential of the investigated strains. These results indicate that effective strains which were isolated from dry bean roots had potential for use as inoculants on beans (Phaseolus vulgaris L.). However, Rhizobium strains should be genetically identified before they are being subjected to recommend as commercial inoculations.

Keywords: Native Rhizobium, inoculation, bean, nitrogen fixation, yield, yield components