Identification of amplified fragment length polymorphism (AFLP) fragments linked to soybean mosaic virus resistance gene in Glycine soja and conversion to a sequence characterized amplified regions (SCAR) marker for rapid selection

Abstract

Soybean mosaic virus (SMV) is a prevalent pathogen that cause yield losses and reduce seed quality in soybean production areas worldwide. Glycine soja is the wild ancestor of domesticated soybean and a potential source of new SMV resistance genes (Rsv). In this study, we applied a combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) to identify molecular markers linked to the Rsv gene in G. soja for resistance to  SMV strain N3 (the most virulent strain) from Northeast China, then a population of 207 F₂ individuals derived from the cross of the SMV resistant line SRY8 (G. soja) with the susceptible line Hefeng25 (Glycine max) for linkage analysis. One dominant AFLP marker, P36M65, was mapped to a position 5.6 cM from Rsv and specific polymerase chain reaction (PCR) primers were designed to convert P36M65 into sequence characterized amplified region (SCAR) maker. This new marker may be useful for marker-assisted breeding of RSV in G. soja.

Keywords: Soybean mosaic virus (SMV), resistance, Glycine soja, amplified fragment length polymorphism (AFLP), sequence characterized amplified region (SCAR)