Effect of different concentrations of carbon source, salinity and gelling agent on in vitro growth of fig (Ficus carica L.)

Abstract

Three in vitro experiments were conducted to study the effect of different concentrations of carbon source, salinity and gelling agent on number of new shoots, shoot length, fresh weight, and dry weight of fig (Ficus carica L.) to develop an efficient in vitro propagation method. The results show that there were no significant differences between the three carbon sources regarding number of newly formed shoots and their shoot lengths. However, there were some differences regarding fresh and dry weights. On the other hand, increased salinity concentrations reduced the number of the newly formed shoots, as well as, their shoot lengths. In addition, liquid media produced more shoots compared with the three solidifying agents used. However, there were significant differences among the concentrations of solidifying agents used.

Keywords: Carbon source, salinity, gelling agent, fig (Ficus carica L.).

African Journal of Biotechnology Vol. 12(9), pp. 936-940,