Identification and genetic characterization of phenol-degrading bacterium isolated from oil contaminated

Abstract

A phenol-degrading novel monosodium glutamate was isolated from oil contaminated soil in India. This strain was found capable to utilize and tolerate up to 9.5 mM of phenol. Based on the results of phylogenetic similarity of 16S recombinant ribonucleic acid (rRNA) gene sequences and fatty acid analysis, strain MSG8 was identified as a novel Acinetobacter sp. The sequence of the partial largest subunit of multicomponent phenol hydroxylase (LmPH) gene of this isolated strain was identified and compared with LmPH gene of Pseudomonas sp.CF600. Reversed phase high performance liquid chromatography showed that the isolate can degrade phenol through catechol ortho fission pathway. In this paper, we reported about the new strain of Acinetobacter sp. capable of degrading phenol (9.5 mM of phenol concentration) under normal conditions. Therefore, strain MSG8 can potentially be used in bioremediation of phenol. The given data demonstrates that the isolated strain exhibits the characteristics of an efficient phenol-degrading microorganism.

Keywords: Phenol, degradation, monosodium glutamate, bioremediation, Acinetobacter sp.

African Journal of Biotechnology Vol. 12(8), pp. 791-797