Effect of different combinations of 6-benzyl amino purine and naphthalene acetic acid on multiple shoot proliferation of plantain (Musa spp.) cv. Matoke from meristem derived explants

Abstract

This study was conducted at the Amhara Region Agricultural Research Institute Tissue culture Laboratory, Ethiopia from April to June 2012 to investigate the effect of different concentrations of 6-benzyl amino purine (BAP) and naphthalene acetic acid (NAA) on virus free plant regeneration and shoot multiplication. The culture meristem grew into callus after 28 days then adventitious plantlets were developed. Among the different concentrations, 5 mg/l BAP + 1.0 mg/l NAA showed highest shoot proliferation (1.00, 1.67, 1.75 and 3.08 shoots per clump) at 10, 20, 30 and 60 days after induction (DAI), respectively. Good numbers of shoots were achieved at 5 mg/l BAP + 0.5 mg/l NAA at 60 DAI (3.08). The longest shoot was produced by the concentration of 5 mg/l BAP + 1.0 mg/l NAA showed (0.43, 2.42, 2.63 and 3.42 shoots per plantlet) at 10, 20, 30 and 60 DAI, respectively. The maximum number of leaves (1.67, 2.67, 3.67 and 4.33 leaves per explant) at 10, 20, 30 and 60 DAI produced on the medium supplemented with 5.0 mg/l BAP and 0.50 mg/l NAA. The lowest number of leaves was obtained from the control treatment. The longest leaves were produced by the concentration of 5.0 mg/l BAP + 1.0 mg/l NAA (1.52, 2.27, 2.70 and 3.13 cm) at 10, 20, 30 and 60 DAI, respectively.

Keywords: Plantain, regeneration, micro propagation

African Journal of Biotechnology Vol. 12(7), pp. 709-719