Identification of single nucleotide polymorphism of growth hormone gene exon 4 and intron 4 in Pesisir cattle, local cattle breeds in West Sumatera Province of Indonesia

Abstract

The pupose of this study was to identify genetic polymorphisms of bovine growth hormone gene exon 4, and intron 4 in local cattle breeds in West Sumatera Province of Indonesia. DNA was isolated from 60 blood samples and polymerase chain reaction (PCR) product of GH5 fragment (366 bp) were directly sequenced. Multiple alignments, including 60 bGH DNA sequences obtained by direct sequencing and bGH sequences from a public database (National Center for Biotechnology Information, acces number M57764), revealed 15 polymorphisms (five SNP, eight deletion, and two insertion). Eight deletions were detected in position 1740, 1743, 1745, 1747, 1749, 1750, 1753, and 1754 with frequency allele of 0.50, 0.22, 0.125, 0.53, 0.06, 0.06 and 0.83, respectively. Besides, two insertions C were detected in position 1790 and 1895 with frequency allele of 0.06 and 1.00, respectively. Five mutations were detected in position 1914, 1930, 1947, 1980, and 2025 with genotypes C → G, G → A, T → G, T → C, and A → G respectively with frequency allele of 0.40, 0.48, 1.00, 0.42, and 0.38, respectively. Another deletion at position 1740, 1743, and 1754 changed codon CAG, TCG, TGG, CTT, GGG, CCC to codon CGT, GTG, GCT, TGG, GCC and new deletion at position 1745, 1749, and 1754 changed the codon TCG, TGG, CTT, GGG, CCC, CTG to TCG, GGC, TGG, GCC, CTG. Deletion at position 1749 and 1754 changed the codon TCG, TGG, CTT, GGG, CCC, and CTG to TCG, TGG, CTG, GGC, CCT. These data provide evidence that GH gene of this breed is slightly different from other breeds. This polymorphic source can be used to refer to performance and to investigate whether these polymorphics are responsible for quantitative variation in growth.

Keywords: Sapi Pesisir, direct sequencing, growth hormone gene, polymorphism

African Journal of Biotechnology Vol. 12(3), pp. 249-252