The earlier identification of the seedless characteristic of the wampee [Clausena lansium (Lour.) Skeels] hybrid by a random amplified polymorphic DNA (RAPD) marker

Abstract

Wampee as an important fruit is widely grown in South China and conventional breeding methods for the production of new seedless wampee cultivars in recent years were operated. Hybridization was
conducted using the seed as female parents and the seedless as male parents. However, the seeds could be gotten from cross combination. An efficient operation was that, non-woven fabrics bag could increase burliness rate in hybridization. In order to earlier identify seedless seedlings in hybrid progeny, the objective of this work is to identify molecular markers linked to seedless trait through bulked segregant analysis. Two bulks, seedless and seed, were prepared by pooling DNA of individual identified plants and used in polymerase chain reaction (PCR) analysis. Out of the 100 primers tested, only S06 primer could get a specific 930 bp restriction random amplified polymorphic DNA (RAPD
amplicon to seedless bulk. As a result, a hybrid was tested with this primer in two cross combinations.

Keywords: Earlier identification, seedless wampee, bulked segregant analysis, randomly amplified polymorphic DNA