Effects of combination of ethylenediaminetetraacetic acid and microbial phytase on the serum concentration and digestibility of some minerals in broiler chicks

Abstract

This experiment was conducted to evaluate the combined effects of ethylene diamine tetraacetic acid (EDTA) and microbial phytase (MP) on the serum concentration and digestibility of some minerals in broiler chicks. This experiment was conducted using 360 Ross-308 male broiler chicks in a completely randomized design with a 3×2 factorial arrangement (0, 0.1 and 0.2% EDTA and 0 and 500 FTU MP). Four
replicates of 15 chicks per each were fed dietary treatments which included; P-deficient basal diet [0.2% available phosphorus (aP)] (NC), NC + 500 FTU MP per kilogram of diet, NC + 0.1% EDTA per kilogram of
diet, NC + 0.1% EDTA + 500 FTU MP per kilogram of diet, NC + 0.2% EDTA per kilogram and NC + 0.2% EDTA + 500 FTU MP per kilogram of diet. The concentration of zinc, copper and manganese of serum and their digestibility and also digestibility of apparent metabolizable energy (AME_n) was evaluated. The results showed that phytase supplementation of P-deficient diets significantly increased zinc concentration of serum (P < 0.05). Interaction effect of EDTA+MP on serum concentration of copper and manganese and also digestibility of zinc was significant (P < 0.05). EDTA supplementation of P-deficient diets significantly increased manganese digestibility in broiler chicks (P < 0.01). 

Keywords: Ethylene diamine tetraacetic acid, microbial phytase, zinc, copper, manganese