Characterization of pathogen responsible for infection in bhindi plant by using phytoplasma specific universal primers

Abstract

Infection in bhindi plant (Abelmoschus esculentus, Family-Malvaceae) is very common in Gorakhpur district of Eastern U.P. region of India. To detect the pathogen, genomic DNA was isolated from the infected bhindi plant sample and amplified through polymerase chain reaction (PCR) by using P1 forward primer and Tint reverse primer, which were phytoplasma specific universal primers. Obtained PCR amplicons were cloned and sequenced. BLAST analysis discovered their 91% identity with the members of ‘Uncultured bacterium’. Phylogenetic tree also make their relationship with uncultured bacterium. Obtained nucleotide sequence was submitted to NCBI through accession number KF663567. To the best of our knowledge, this is the first report of “uncultured bacterium” (473 bp), from Gorakhpur district of Eastern U.P. region of India, associated with infection in bhindi plant. 

Keywords: Abelmoschus esculentus, Gorakhpur district, Uncultured bacterium, Acc. No. KF663567.

African Journal of Biotechnology, Vol 13(51) 4606-4611