Seed salt-soluble protein expression as marker of local Medicago ciliaris populations adapted to highly salted region from Algeria (Oran Great Sebkha)

Abstract

The present study estimated the diversity of seed storage salt-soluble proteins of Algerian populations of Medicago ciliaris species, for understanding biochemical and molecular features involved in response during plant stress adaptation. Eleven patterns of M. ciliaris populations with two moderate sensitive ecotypes to NaCl, two reference genotypes and seven prospecting populations near and far from a strongly salted area (Sebkha of Oran) were investigated by one dimensional electrophoresis SDS-PAGE. The results show that the proteins profiles were very similar with 10 to 12 bands profiles about all populations, after electrophoresis in denaturing conditions; but an 80 kDa band was visible only on population from Sebkha origin, where the salt content in the soil is the highest one. It appears that, this globulin protein is related to salt tolerance and could be used as SDS-AGE markers for differentiating between tolerating and sensible M. ciliaris populations to salt stress.

Keywords: Oran Great Sebkha, annual Medicago ciliaris, seed storage salt-soluble proteins, sodium dodecyl sulfate- poly acrylamide gel electrophoresis (SDS-PAGE) markers.

African Journal of Biotechnology, Vol 13(47) 4355-4360