Effect of equation pro and kema zed fungicides on cellulase and pectinase enzymes produced by some phytopathogenic fungi of broad bean

Abstract

Two fungicides (equation pro and kema zed) were added to the medium in five doses (50,100, 200, 300 and 400 ppm active ingredient) to investigate the chemical control of cellulase and pectinase enzymes produced by some plant pathogens of broad bean. Alternaria alternata, Alternaria citri, Alternaria and Cochliobolus spicifer were isolated from diseased leaves of broad bean. The incorporation of these fungicides into the media for cellulase and pectinase enzymes exhibited an inhibitive effect on both cellulase and pectinase enzymes by all doses used except in few cases; the production of exo- and endo-β-1, 4-glucanase enzymes were slightly increased compared to the control at 50 and 100 ppm doses. The inhibitive effect of equation pro on endo- and exo-β-1,4-glucanase production ranged between 5.9 to 70.4% by A. alternata, A. citri and C. spicifer. However, kema zed exhibited an inhibitive effect on cellulases which ranged between 1.9 to 51.9%. On the other hand, the inhibitive effect of Equation pro on pectinase enzyme ranged between 20.1 to 75.6% by A. citri and A. raphani while kema zed exhibited an inhibitive effect ranged between 13.5 to 62.8%. The inhibitive effect of these fungicides on the mycelial growth of tested fungi was nearly similar to those for the enzyme production.  

Keywords: Fungicides, cellulase and pectinase enzymes, broad bean fungi.

African Journal of Biotechnology, Vol 13(46) 4330-4337