Pathogenicity and proteome production of Isaria fumosorosea (=Paecilomyces Fumosoroseus) (WISE) isolates against lemon butterfly, Papilio demoleus (Papilionidae: lepidoptera)

Abstract

The pathogenic potential and catalytic triad conserved amino acids of the isolates Isaria fumosorosea (=Paecilomyces fumosoroseus) (Ifr₁ and Ifr₂) in response to Papilio demoleus was analysed. The isolates showed its potential in killing P. demoleus causing mortality of 72.23 and 61.90% at the end of 8 days with 10⁸ spores ml^-1 concentrations. The enzyme assays (higher proteolytic and chitinolytic activity) also showed that the Ifr₂ was more efficient than Ifr₁. The predictions of catalytic triads (serine, histidine and asparagine) were also visualized in the peak level obtained in infra-red (IR) and H₁ nuclear magnetic resonance (NMR) spectra. With this information it was suggested that, partial characterization of catalytic domain was predicted in the fungal isolates Ifr.

Keywords:  Entomopathogenic fungi, Isaria fumosorosea, Papilio demoleus, biological control.

African Journal of Biotechnology, Vol 13(43) 4176-4182