Molecular characterization of Doom pigs using microsatellite markers

Abstract

Assessment of genetic diversity was carried out in Doom pig using 22 microsatellite markers, recommended by Food and Agriculture Organization (FAO). All the studied loci were highly polymorphic and a total of 120 alleles were observed across the investigated loci. The range of alleles was found to be 4 to 10 with a mean of 5.4±1.65. The frequency distribution of microsatellite alleles in the population was from 0.02 to 0.6667. The observed and expected heterozygosity values were 0.62±0.287 and 0.67±0.142, respectively. The polymorphic information content (PIC) was 0.63±0.143. Microsatellite analysis revealed moderate to less genetic diversity in the Doom pig population. The overall mean of within-population inbreeding estimate (F_IS) was 0.089. The Shannon’s information index (I) was sufficiently high with a mean of 1.36. The bottleneck analysis revealed that population has not undergone any recent reduction.

Keywords: Heterozygosity, microsatellites, polymorphic information content (PIC), bottleneck, doom pig

African Journal of Biotechnology, Vol 13(30), 3017-3022