Analysis and identification of oils from seed extract of Anthonotha macrophylla using gas chromatography-mass spectrometry (GC-MS)

Abstract

The volatile components obtained from the seed extract of Anthonotha Macrophylla were analyzed using gas chromatography-mass spectrometry (GC-MS). Prior to GC-MS analysis, Soxhelt extraction was carried out from the seeds of A. Macrophylla. Agilent GC-MS system comprising 6890GC model coupled with 5973 n mass selective detector was used for analysis. The GC is equipped with Agilent 7673 autosampler and a 30 m 0.25 id DB-1 MS dimethylpolysiolaxane capillary column. The MS source temperature was set at 230C and electron energy at 70V. The ionisation mode was electron ionization and the mass range was 50 to 550 while the scan time was 1 scan/min. The different compounds were identified by matching their mass spectra with the MS spectra in the NIST library. Various compounds were separated and identified but eight of these were at an elevated level. These include: n-hexadecanoic acid, n-octadecadienoic acid; cis-vaccenic acid; octadecanoic acid; hexadecanoic acid 2-hydroxy-1-(hydroxymethyl) ethyl ester; campesterol; stigmasterol and gamma-sitosterol.

Keywords: Gas chromatography-mass spectrometry, determination, evaluation, composition, Anthonotha macrophylla, seed

African Journal of Biotechnology, Vol 13(22) 2260-2264