Identification and utility of sequence related amplified polymorphism (SRAP) markers linked to bacterial wilt resistance genes in potato

Abstract

Bacterial wilt caused by Ralstonia solanacearum is one of the most economically important diseases affecting potato (Solanum tuberosum). It is necessary to develop more molecular markers for potential use in potato genetic research. A highly resistant primitive cultivated species Solanum phureja was employed to generate a F1 mapping population to perform the bulked segregant analysis (BSA) for screening and identifying of sequence related amplified polymorphism (SRAP) markers linked to the potato resistance to bacterial wilt. A linkage map containing 23 DNA markers distributed on three linkage groups, and covering a genetic distance of 111 cm with an average distance of 5.8 cm between two markers was developed. Two SRAP markers, Me2em5 linked in repulsion phase and Me2em2 in coupling phase, flanked the resistance genes at genetic distances of 3.5 and 3.7 cm distance, respectively. These markers and two others were used for early seedling selection in a BC1 population. The results show that this marker system could be used in marker assisted selection (MAS) breeding program.

Keywords: Sequence related amplified polymorphism (SRAP) marker, potato, bacterial wilt

African Journal of Biotechnology, Vol 13(12), 1314-1322