Virulence, serotype and phylogenetic groups of diarrhoeagenic Escherichia coli isolated during digestive infections in Abidjan, Côte d’Ivoire

Abstract

The virulence, serotype and phylogenetic traits of diarrhoeagenic Escherichia coli were detected in 502 strains isolated during digestive infections. Molecular detection of the target virulence genes, rfb gene of operon O and phylogenetic grouping genes Chua, yjaA and TSPE4.C2 was performed. Prevalence of strains harbouring virulent genes was 7.8%. The virulent genes eaeA, bfp, stx2, st1, lt, aggA, east1, ipaH, ial, cnf1 and afa were detected. EAEC (36%) and both EPEC and ATEC (25.6%) are the most detected pathovars (p<0.05). STEC (5.1%), NFEC (7.7) and DAEC (7.7) are less represented. Serogroups are overall diversified (89%), however, serogroups O157, O103 and O86, previously known to be associated with virulence were revealed. Most of the E. coli pathovars (53%) belonged to phylogenetic group A and in decreasing importance order, to D (23.5%), B1 (11.7%) and B2 (11.7%) groups. The study shows a diversified population of intestinal strains (84.6%), with a low phenotypic and phylogenetic link lower (p<0.05). Due to the great diversity of pathotypes, continuous monitoring should be implemented to identify risk factors and major pathways of contamination that help defining strategies to reduce infections associated with E. coli.

Keywords: Escherichia coli, virulence gene, serogroup, phylogenetic group, diversity

African Journal of Biotechnology, Vol. 13(9), pp. 998-1008, 26 February, 2014