Highly efficient in vitro adventitious shoot regeneration of Adenosma glutinosum (Linn.) Druce using leaf explants

Abstract

Adenosma glutinosum (Linn.) Druce is an important aromatic plant, but no information is available regarding its regeneration, callus induction and proliferation from leaf explants. In this study, an in vitro shoot regeneration procedure was developed for native A. glutinosum using leaf explants. Callus induction and shoots regeneration from leaf explants was evaluated on Murashige and Skoog (MS) media supplemented with combinations of 6-benzylaminopurine (6-BA) and α-naphthaleneacetic acid (NAA). Callus induction in all 16 treatments exceeded 95%, and the highest adventitious shoot number per callus (7.22 shoots per explant) was obtained when leaf explants were cultured on MS medium supplemented with 0.5 mg• L^-1 6-BA, 0.1 mg• L^-1 NAA, 3% sucrose and 0.72% agar. The highest shoots strengthening were obtained when adventitious buds were cultured on half-strength MS medium supplemented with 0.3 mg• L^-1 NAA, 3% sucrose, 1.0 g• L^-1 active carbon and 0.72% agar. The highest total root number (45.2) and root length (43.3 cm) were obtained when adventitious buds were cultured on half-strength MS medium supplemented with 0.0 mg• L^-1 NAA, 3% sucrose, 1.0 g L⁻¹ active carbon and 0.72% agar, while the highest total root surface area (4.1 cm²) and total root volume (114.1 mm³) were obtained when adventitious buds were cultured on half-strength MS medium supplemented with 0.5 mg• L^-1 NAA, 3% sucrose, 1.0 g• L^-1 active carbon and 0.72% agar. The efficient plant regeneration system developed here will be helpful for rapid micropropagation and further genetic improvement in A. glutinosum.

Keywords: Adenoma glutinous, plant growth regulator, plant regeneration

African Journal of Biotechnology Vol. 11(29), pp. 7542-7548, 10 April, 2012