Full-length enriched multistage cDNA library construction covering floral bud development in Populus tomentosa

Abstract

Flowering involves expression of a suite of genes associated with floral development. The genome of the Chinese white poplar (Populus trichocarpa) was sequenced because of its importance as a model tree for genetic studies as well as being an economically important woody plant. However, information on expressed genes involved in poplar floral bud development is insufficient to allow annotation of genes and use of the genomic information. To isolate and characterize genes involved in flowering of Populus tomentosa, floral bud samples were collected at different developmental stages from floral bud initiation to flower maturity, and full-length enriched cDNA libraries from both male and female floral buds were constructed. The results of titer analysis showed that the titer of the female and male primary libraries were 8.00 × 10⁵ and 7.20 × 10⁵ pfu/ml, respectively, and the titer of the amplified libraries were 2.60 × 10⁸ and 2.56 × 10⁸ pfu/ml, respectively. The combination ratio reached 90% and the insert size was 400 to 2000 bp. The results indicated that cDNA libraries were successfully constructed.

Keywords: cDNA library, floral bud, flowering, Populus tomentosa

African Journal of Biotechnology Vol. 11(29), pp. 7373-7377, 10 April, 2012