Cytosolic phosphoenolpyruate carboxykinase (PEPCKC) gene polymorphism in three chicken breeds

Abstract

Phosphoenolpyruate carboxykinase (PEPCK) gene is an enzyme which has a key role in gluconeogenesis. In the chicken genome, there are two different types of PEPCK gene: mitochondrial PEPCK (PEPCK-M) and cytosolic PEPCK (PEPCK-C). In this study, 150 random samples from two native populations in Sistan and Baluchistan Province, namely, Khazak and Dashtiari, and Ross chicken were analyzed. DNA was extracted from feather pulps and PCR was carried out using appropriate primers (F2R2), which amplifies a 1000 bp fragment from the chicken PEPCK-C gene. The fragment covers from promoter to exon 2 of the gene. PCR products were then digested by restriction enzymes BstEII and ACil. Allelic as well as genotypic frequencies were determined and analyzed by Popgene software. The allelic frequencies BstEII for Khazak sample were A = 0.975 and B = 0.025; for Dashtiari sample; A = 0.95 and B = 0.05 and for Ross samples, A = 0.94 and B = 0.06. The allelic frequencies digestion by ACil for Khazak sample were A = 0.86 and B = 0.14; for Dashtiari sample, A = 0.98 and B = 0.02 and for Ross samples, A = 0.95 and B = 0.05. Haplotype analysis of two single nucleotide polymorphism (SNP) showed that there were four alleles and genotypes. The present results indicated that little polymorphism exists in the samples for this locus.

Key words: Polymorphism, PEPCK gene, Sistan and Baluchistan native chicken, Khazak, Dashtiari.