Effect of storage media and time on fin explants culture in the goldfish, Carassius auratus

Abstract

The effect of storage media and time was investigated on fin explants culture in the goldfish (Carassius auratus). Fin explants under sterile conditions were able to produce cells at different storage media and time. On the outgrowth of cells, fin explants stored for seven days before culturing showed significantly higher growth (P<0.05) as observed on the fin explants stored in Dulbecco’s modified Eagles medium (DMEM, 84.44%), phosphate buffered saline (PBS, 62.42%) and in control/fresh fin explants (100%), compared with the explants with no storage medium (25.56%) at day three of culture. From day seven to 14, all caudal fin explants exhibited a 100% outgrowth of cells regardless of treatment. However, caudal fin explants kept for 10 days in 4°C showed a significant difference (P<0.05) from fin explants stored in DMEM (96.67%), PBS (75.57%) and the control (100%), as compared with no storage medium (no growth) on day three. Moreover, only 6.67% of no storage medium group could maintain outgrowth of cells, while other treatment group reached 100% of outgrowth after 14 days of culture.

Key words: Carassius auratus, fin explants, goldfish, storage.