Studies on the intiation of callusing and regeneration of plantlets in three different basal media with varied plant growth regulators for the micropropagation of Anthurium scherzeriaum using leaf and spathe as explants

Abstract

Anthurium scherzeriaum is one of the most common ornamentals used in floriculture industry. However, there is shortage of planting material supplied to the cultivators. To overcome this problem, many breeders adopted micropropagation techniques. The usage of high dosage of plant growth regulators in the media resulted in mutation and loss of potentiality in the regeneration. An attempt was made to derive a suitable medium and selection of proper explants to avoid undesirable results. Explants like young old leaf segments and spathe were used. Three different basal medias such as Murashige and Skoog (MS), Nitsch and White’ media were used with varying concentration of growth regulators. The obtained results indicated marked improvement in the growth pattern and morphological features of the plants obtained by culturing spathe segments in Nitsch’ medium.

Key words: Anthurium scherzeriaum, tissue culture, floriculture, ornamental plants