Effect of excess dietary copper on proliferation and differentiation of the proerythroblasts and erythrocytes in rats

Abstract

This research was carried out to test the cytotoxic effects of excess copper in rats. Animals were divided into three groups, each containing five animals. Low dose (2 mg/kg) and high dose (4 mg/kg) of copper sulphate were force-fed into the animal by a stomach tube daily for 3 weeks and the third group was used as the control. At the end of each week, three animals (one of each group) were randomly selected and sacrificed. Blood samples were collected and blood smears were made. The bone marrow was collected from the heads of long-bones and bone marrow smears were also prepared. It was found that the application of copper sulphate doses modulates the proliferation and differentiation of stem cell progenitors and erythrocytes. Several alterations were observed and these were time- and dosedependent. Of these alterations, the predominant existence of giant pro-erythroblasts and promyeloblasts marked the increase of adipose cells and degeneration of pro-erythroblasts among the bone marrow cells. Also observed were hypochromia, anisocytosis, fragmentation and burr-shaped erythrocytes.

Key words: Environmental pollution, copper toxicity, stem cells, blood, rats.