Production and evaluation of doubled haploid lines of barley via detached-tiller culture method

Abstract

This research was conducted to compare the classic (C) and detached-tiller methods (a: sterile by clip and scissors D₁; b: sterile by hot water D₂) for producing haploids in barley. The parental materials used in this research were F₁ four genotypes of Hordeum vulgare: B₁: Reihane × Legia; B₂: Reihan × Igri; B₃: Kavir × Igri; B₄: Kavir × Legia, and the Hordeum bulbosum was PB1 genotype (Plant Breeding Institute). The traits such as seed set percentage, embryo development, haploid seedling development and produced doubled haploid lines were analyzed statically using χ² test. The comparison was made among arable barley B₁, B₂, B₃, B₄ crossing wild barley of H. bulbosum. In C approach, there was no significant difference concerning the percent of forming seed, embryo and haploid production. While in D₁ approach, there was a significant difference in embryo forming percent but no significant difference was observed for haploid plant and seed set percent. Moreover, in D₂ approach, there was a significant difference for seed set percent. However, for haploid production and embryo development percent, the approach D₁ in regards with the percent of forming embryo and production of haploids was superior to the other two approaches, C and D₂.

Key words: Detached-tiller culture, haploid, doubled haploid, Hordeum bulbosum, Hordeum vulgare.