Performance of polymer compositions as carrier to cowpea rhizobial inoculant formulations: Survival of rhizobia in pre-inoculated seeds and field efficiency

Abstract

Rhizobial-legume association is a symbiosis that is exploited in agriculture by inoculant production. Efficient inoculant formulations are needed to increase the biological nitrogen fixation rates in agroecosystems. The aim of this work was to evaluate inoculant formulations to cowpea (Vigna unguiculata L. Walp) containing polymers as carrier materials and rhizobial strains. To assess the efficiency of the new inoculants formulations, the rhizobial survival in inoculated and stored seeds and the field performance of a selected formulation were evaluated. The rhizobial survival was higher in cowpea seeds inoculated with MgO compatibilized polymeric formulations, compared to standard peatbased inoculant and compatibilized polymeric formulations compatibilized with ZnO. Seeds inoculated with IPC 2.2 formulation presented more than 10⁶ cells per seed through 35 days of storage with low contamination levels. The field performance of the polymeric compositions was the same as that observed in the peat-based inoculant treatments. Among the three rhizobial strains isolated, two of them were able to provide the same shoot nitrogen content and productivity in comparison to the recommended strain. These results indicate the feasibility of inoculants with the polymer blends as carrier materials and the efficiency of the new fast growing rhizobia from Brazilian semi-arid.

Key words: Inoculant technology, strain selection, biological nitrogen fixation.