Differential expression of ATP-dependent RNA helicase gene in viable but nonculturable Salmonella pullorum

Abstract

The purpose of this study was to screen new gene involved in viable but nonculturable (VBNC) Salmonella using messenger ribonucleic acid (mRNA) differential display. Strain cultured on liquid lysogeny broth (LB) medium at 4°C was induced for VBNC state; activated genes were detected using mRNA differential display and differential fragment of adenosine triphosphate (ATP)-dependent RNA helicase rh1B gene was verified. The fragment coded adenosine triphosphatase (ATPase) structure domain of DEAD-box helicase. The screened fragment which codes ATPase structure domain of DEAD-box helicase may be a candidate marker for VBNC Salmonella detection.

Key words: Viable but nonculturable, Salmonella, ATP-dependent RNA helicase.