Study of citrullinaemia disorder in Khuzestan Holstein cattle population of Iran

Abstract

The present study investigated the occurrence of autosomal recessive genetic disease, citrullinaemia, in Khuzestan native cows and Iranian Holstein cattle. Genomic DNA was isolated from the blood of the cows (n = 330). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was performed to identify carriers of this disease. Then to determine existence of mutant citrullinaemia allele, all cows were confirmed by DNA sequencing. This study showed that none of the cows were carriers or mutants of citrullinaemia deficiency. More attention is necessary to check bulls related to possibility of being citrullinaemia carrier. This is important for economical reason and citrullinaemia mutation and its recessive hereditary disorder. However, no carrier was observed in this study, and for more detection of genetic disorders, it seems that a wide screening program is needed.

Key words: Citrullinaemia, sequencing, cattle, Iran.