In vivo anti-inflammatory effects of anti-toxic principles from Moringa oleifera(MO11) and Musa sapientum (MS06) via NF-KB/IL-4/IL-10- mediated pathway in Cadmium Chloride-induced hepato-toxicity.

Abstract

Cadmium-induction of hepato-toxicity and inflammation in animal models have been reported. The mechanism underlying hepatic inflammation is poorly understood. This study evaluated antiinflammatory potentials of MO11 (isolated from Moringa oleifera leaves) and MS06 (isolated from Musa sapientum suckers) in Cadmium Chloride (CdCl)-induced hepato-toxicity and inflammation in rats.Twenty-eight adult male wistar rats (average weight of 155 g) were randomly divided into 7 groups (n = 4). Group 1 was control. Groups 2 - 4 and 7 received single intraperitoneal administration of 1.5 mg/Kg bodyweight of CdCl on Day 1. Thereafter, Groups 3, 4 and 7 were post-treated with 15 mg/Kg bodyweight of MO11, 15 mg/Kg bodyweight of MO11 + 7 mg/Kg bodyweight of MS06 and single-dose of 3.35 mg/Kg bodyweight of Doxorubicin respectively (Days 1 - 17). Groups 5 and 6 received only MO11-dose and Olive Oil (vehicle) respectively (Days 1 - 17). Liver histopathology (Heamatoxyline and Eosin technique) and ELISA concentrations of proinflammatory biomarkers (IL-1β, IL-6, IL-8 and NF-kB) and anti-inflammatory biomarkers (IL-4 and IL-10) in liver homogenates of rats of Groups 1 - 7 were evaluated. Computed data were statistically analysed using Mann-Whitney-U test at P ≤ 0.05. Histo-pathological analyses showed normal liver histology in Groups 1 - 7. Post-treatments of CdCl-induced hepato-toxicity and hepatic inflammation with MO11 and MO11+MS06 resulted in downregulations of IL-1β, IL-6, IL- 8 and NF-kB, but upregulations of IL-4 and IL-10 in Groups 3, 4 and 7, compared with Group 2. MO11 and MS06 possess hepato-protective and anti-inflammatory potentials.