Survey of plant parasitic nematodes and disease severity of common bean lines evaluated for reaction to root knot nematodes infestation

Abstract

Plant parasitic nematodes are important pests in crop production in sub-Saharan Africa. The objective of this study was to identify the occurrence of nematodes associated with common bean (Phaseolus vulgaris L.) and evaluate breeding lines for their reaction to Meloidogyne spp. in Ghana. Common bean rhizosphere soil was sampled and processed using Modified Baermann Tray method. Five nematode genera, namely Meloidogyne, Pratylenchus, Rotylenchulus, Helicotylenchus and Trichodorus were extracted. The first four genera listed above were prevalent across locations, with Trichodorus present in 30% of the fields sampled. The highest nematode population density of 319 juveniles per 200 cubic centimeter of soil was recorded for Meloidogyne spp. compared to 45 juveniles per 200 cubic centimeter, for Trichodorus. Twelve breeding lines were evaluated by inoculating roots of two-weeks-old plants with 2000 infective-stage juveniles of Meloidogyne sp. Reactions of test lines to Meloidogyne sp. infection were assessed by determining the number of egg masses and galling index (GI) on roots. Reproduction index (RI) was used to classify test lines as resistant or susceptible. Significant differences (P < 0.05) were observed in the number of eggs, GI and RI among lines tested. No resistant line was identified; however, lines SEF 47, BFS 35 and BFS 60 were moderately resistant, with RI of 13.1, 17.4 and 23.7%, respectively. Line SEF 60, although classified as slightly resistant, recorded a 100 seed weight of 26.0 g, which was 60% higher than line SEF 53 with seed weight of 16.2 g. Moderately resistant common bean lines identified could be used in common bean improvement programmes to develop elite cultivars tolerant to root knot nematodes.