Distribution of potato viruses in Uganda

Abstract

Viral diseases are among the major factors affecting potato (Solanum tuberosum L.) production in Uganda. Knowledge of the types of viruses and their distribution levels in the country is vital for targeting sound management strategies. The objective of this study was to assess the prevalence and distribution of potato viruses in different potato growing areas in Uganda. Virus diagnostic surveys were conducted across the main potato producing regions and virus detection was done using DAS-ELISA and RT-PCR techniques. The study showed that the most widely distributed and economically important potato viruses were: PVS (31-90.9%), PLRV (2.6-81.3%), PVY (7.1-54.5%) and PVX (8.3 -53.3%); while the least detected viruses were PVM (18.2%) and PVA (9%). The viruses were prevalent either singly or in mixtures. Up to 42.8% of the samples were infected with one virus, 20.9% with two viruses and 4.2% with three viruses; while no virus was detected in 31.3% of the samples. Of the double infections, the PVY+PVS combination was the most common and widely distributed (2.1-18.2%) in 12 districts; followed by PVY+PLRV (1.8-21.3%) occurring in six districts, PVM+PVS (7.9-16.7%), PLRV+PVX (2.4-14.3%) in 5 districts and PVY+PVX (2.4-4.4%) in 3 districts. Triple infections involving PVY+PLRV+PVS were recorded at prevalence levels of 2.2-18.6% in six district. Altitude, temperature, varieties and seed sources showed relationships with the variation in the prevalence of the viruses. There were high virus prevalence and disease severity levels in low altitude areas (1088-1334 m.a.s.l) of mid-western sub-region (Mbarara and Lwengo), Central (Kibaale, Mubende) and Mid Northern (Pader) sub region compared to high altitude areas (>1600 m.a.s.l). The Virus risk Area Modeling results showed the largest area (48.6%; 1,308,160 ha) at moderate risk of virus infection; while 27.3% (732,305 ha) was at high risk of virus infection. Based on the distribution level of the viruses, potato production could be intensified in areas with less virus pressure mainly, in parts of West Nile and Rwenzori regions.