QTL analysis for early yield in a pseudo F2 population of cassava

Abstract

Genetic mapping of early bulking in a full-sib population of cassava was continued in a selfed family of 268 cassava plants derived from a single progeny of the full-sib  population. The pseudo F₂ population was analysed with 122 segregating SSR  markers. A previously constructed linkage map of cassava consisting of 22 linkage groups covering 1236.7 eM, with an average marker distance of 18 eM was used for this study. The F₂ population was evaluated for components of early yield, namely, dry root yield (DR) at 7 months, harvest index (HI), and weight of fresh foliage (FF). Interval mapping, with single- and two-QTL  models, was used to identify QTLs. The single-QTL model identified three QTLs each for DR, FF, and HI. The two-QTL model approach  identified groups of QTLs that together explained 33% for FF, 43.5% for DR and 36% for HI. The identification of QTLs involved in early yield is an important step toward understanding quantitative genetic variation of early yield and implies reconsideration of breeding strategies for improvement of this complex trait.

Key words: Linkage map, molecular marker-assisted selection, SSR markers