Morphometric and histopathological evaluation of selected organs of albino rats following subcutaneous administration of Acalypha wilkesiana leaf extract

Abstract

Acalypha wilkesiana (Copperleaf) belongs to the family (Euphobiaceae), commonly found in the coastal areas of Nigeria. It is used for the treatment of skin infections and other ailments. The study is designed to examine the morphometric and histopathology of selected organs of albino rats following subcutaneous administration of the leaf extract. Twenty-five albino rats of both sexes with average weight of 205.20g were selectively assigned to four treatment groups and a control group (n=5). The rats were housed in plastic cages and allowed adequate access to feed and water ad libitum. The plant was identified and authenticated while the crude leaf extract was prepared and administered at dose range (50 to 200mg/kg) b. w. They were sacrificed at the end of the experiment, grossing was done shortly after excision of selected organs and was fixed in 10% neutral buffered formalin. Decrease in body weights of the treated rats was statistically significant (p < 0.05). Morphometric and histopathology findings indicated normal morphology of the skin, muscles, liver and kidneys examined. Therefore, the plant decoction may be used as herbal remedies. However, dose regimen still calls for concern.

Key word: Copperleaf, Rattus novergicus, morphometric and histopathology analyses